Effect of tetracyclines on collagenase activity in patients with recurrent aphthous ulcers

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Abstract
Human neutrophil‐type (MMP‐8) and fibroblast‐type (MMP‐1) interstitial collagenase, and their inhibition by tetracyclines in saliva from patients with recurrent aphthous ulcers (RAU) or aphthae, were studied by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and enzymological analyses. In the salivary specimens obtained from patients with aphthae, collagenase was found in endogenously active form and was predominantly of MMP‐8 type. Topical rinsing treatment with chlortetracycline (Aureomycin®) alleviated the discomfort caused by the lesions but did not reduce salivary collagenase amounts; however in vitro, doxycycline inhibited salivary collagenase totally.