Dimethylarsinic acid causes inhibition of microtubule assembly and inhibition of calcium‐sensitive disassembly of microtubules via interaction with glutathione
- 8 July 2002
- journal article
- research article
- Published by Wiley in Applied Organometallic Chemistry
- Vol. 16 (8) , 432-436
- https://doi.org/10.1002/aoc.326
Abstract
The effects of dimethylarsinic acid (DMAA) on microtubule assembly in vitro were investigated in terms of the role of glutathione (GSH). Increase in turbidity due to assembly of brain tubulins was monitored in the presence or absence of DMAA. DMAA at concentrations up to 10 mM did not cause inhibition of the increased turbidity. GSH at concentrations over 10 mM enhanced the increase in turbidity. By contrast, the increased turbidity was inhibited markedly when DMAA and GSH were combined in the ratio of 5:1. Moreover, the combination similarly inhibited calcium‐induced disassembly of microtubules with a potency equivalent to that of 10 µM taxol. These results suggest that DMAA inhibited not only microtubule assembly, but also calcium‐sensitive disassembly of microtubules, via interaction with GSH. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
This publication has 25 references indexed in Scilit:
- Distribution of γ-tubulin in multipolar spindles and multinucleated cells induced by dimethylarsinic acid, a methylated derivative of inorganic arsenics, in Chinese hamster V79 cellsToxicology, 1999
- Induction of chromosomal aberrations in cultured human fibroblasts by inorganic and organic arsenic compounds and the different roles of glutathione in such inductionMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1996
- Glutathione plays different roles in the induction of the cytotoxic effects of inorganic and organic arsenic compounds in cultured BALB/c 3T3 cellsCellular and Molecular Life Sciences, 1994
- Reduction and binding of arsenate and dimethylarsinate by glutathione: a magnetic resonance studyChemico-Biological Interactions, 1994
- Dimethylated arsenics induce DNA strand breaks in lung via the production of active oxygen in miceBiochemical and Biophysical Research Communications, 1989
- Calcium and the regulation of mitotic eventsTrends in Biochemical Sciences, 1986
- Biotransformation of trivalent and pentavalent inorganic arsenic in mice and ratsEnvironmental Research, 1981
- Methylation of inorganic arsenic by mammalsJournal of Agricultural and Food Chemistry, 1975
- Biological Cycles for Toxic Elements in the EnvironmentScience, 1974
- Methylated Forms of Arsenic in the EnvironmentScience, 1973