Supplemental Copper and Selenium For Calves: Effects Upon Ceruloplasmin Activity and Liver Copper Concentration

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Abstract
The relation between plasma copper, ceruloplasmin concentration, and copper oxidase activity in postnatal calves fed supplemental amounts of copper (100 and 200 p.p.m.) and selenium (0.7 p.p.m.) singly or in combination was studied. Thirty-six 3-day-old calves were assigned at random to six dietary treatments (2 × 3 factorial) with six calves in each treatment. Blood samples were collected biweekly for a period of 56 days.A significant (P = 0.01) positive linear correlation was obtained between plasma copper (μg/100 ml) and p-polyphenylenediamine oxidase activity (expressed as absorbance at 552 mμ). Considerable changes in plasma copper were observed as the level of copper or copper plus selenium in the diet was elevated. Copper or copper plus selenium supplementation did not have any significant effect on the amount of copper in the albumin fraction of plasma and ceruloplasmin activity was completely absent.

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