Biodegradable alginate microspheres as a delivery system for naked DNA.
- 1 April 1999
- journal article
- Vol. 63 (2) , 148-52
Abstract
Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial beta-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.This publication has 23 references indexed in Scilit:
- Elegantly presented DNA vaccinesNature Biotechnology, 1997
- Biologically erodable microspheres as potential oral drug delivery systemsNature, 1997
- Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5Science, 1997
- DNA vaccinesLife Sciences, 1996
- Mechanism of Adenovirus-Mediated Endosome Lysis: Role of the Intact Adenovirus Capsid StructureBiochemical and Biophysical Research Communications, 1994
- Gene Transfer Optimization with Lipospermine-Coated DNADNA and Cell Biology, 1993
- Direct Gene Transfer into Skeletal MuscleIn Vivo: Factors Affecting Efficiency of Transfer and Stability of ExpressionHuman Gene Therapy, 1993
- Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral ProteinScience, 1993
- Genetic immunization is a simple method for eliciting an immune responseNature, 1992
- Direct Gene Transfer into Mouse Muscle in VivoScience, 1990