Pex7p translocates in and out of peroxisomes in Saccharomyces cerevisiae
Open Access
- 15 November 2004
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 167 (4) , 599-604
- https://doi.org/10.1083/jcb.200407119
Abstract
Pex7p is the soluble receptor responsible for importing into peroxisomes newly synthesized proteins bearing a type 2 peroxisomal targeting sequence. We observe that appending GFP to Pex7p's COOH terminus shifts Pex7p's intracellular distribution from predominantly cytosolic to predominantly peroxisomal in Saccharomyces cerevisiae. Cleavage of the link between Pex7p and GFP within peroxisomes liberates GFP, which remains inside the organelle, and Pex7p, which exits to the cytosol. The reexported Pex7p is functional, resulting in import of thiolase into peroxisomes and improved growth of the yeast on oleic acid. These results support the “extended shuttle” model of peroxisome import receptor function and open the way to future studies of receptor export.Keywords
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