Structural Organization of a Hypermethylated Nuclear DNA Component in Physarum polycephalum

Publisher Website
Google Scholar
Abstract
Digestion of P. polycephalum nuclear DNA using the restriction endonuclease HpaII generates 2 components, distinguishable on the basis of their molecular size. The high-MW, HpaII-resistant component, which accounts for 20% of the DNA, contains a 5-fold greater concentration of 5-methylcytosine residues than the low-MW HpaII-digested fraction. Segments of hypermethylated (M+) DNA are largely composed of a single, long, highly repeated sequence, and this major element is sometimes associated with other less highly repetitive sequences in the M+ DNA fraction. Restriction mapping of cloned Physarum M+ DNA segments, and Southern blot analysis of genomic DNA using subcloned segments of M+ DNA as a probe, provide evidence for sequence variation within different copies of the dominant highly repeated element, and possibly the other associated repeats in M+ DNA, and additionally that almost complete tandemly repeated copies of the major repeat are found in some M+ DNA segments.

This publication has 1 reference indexed in Scilit: