Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures: Preparation, bulk and single molecule spectroscopy
Open Access
- 5 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (22) , 5257-5262
- https://doi.org/10.1016/j.febslet.2006.08.049
Abstract
Reconstitution of the 16 kDa N‐terminal domain of the peridinin–chlorophyll–protein, N‐PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N‐PCP. Besides homo‐chlorophyllous complexes, hetero‐chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo‐ and hetero‐chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.Keywords
This publication has 21 references indexed in Scilit:
- Myoglobin with modified tetrapyrrole chromophores: Binding specificity and photochemistryBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2006
- Quantum Chemical Evidence for an Intramolecular Charge-Transfer State in the Carotenoid Peridinin of Peridinin−Chlorophyll−ProteinThe Journal of Physical Chemistry B, 2003
- The 15-kDa forms of the apo-peridinin-chlorophyll a protein (PCP) in dinoflagellates show high identity with the apo-32 kDa PCP forms, and have similar N-terminal leaders and gene arrangementsMolecular Genetics and Genomics, 2001
- Peridinin Chlorophyll a Protein: Relating Structure and Steady-State SpectroscopyBiochemistry, 2000
- Effect of the Solvent Environment on the Spectroscopic Properties and Dynamics of the Lowest Excited States of CarotenoidsThe Journal of Physical Chemistry B, 2000
- Excited State Properties of Peridinin: Observation of a Solvent Dependence of the Lowest Excited Singlet State Lifetime and Spectral Behavior Unique among CarotenoidsThe Journal of Physical Chemistry B, 1999
- Structure-Based Calculations of the Optical Spectra of the Light-Harvesting Peridinin−Chlorophyll−Protein Complexes from Amphidinium carterae and Heterocapsa pygmaeaThe Journal of Physical Chemistry B, 1999
- Structural Basis of Light Harvesting by Carotenoids: Peridinin-Chlorophyll-Protein from Amphidinium carterae Science, 1996
- [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranesPublished by Elsevier ,1987
- A RAPID AND EFFICIENT METHOD TO PREPARE CHLOROPHYLL A AND B FROM LEAVESPhotochemistry and Photobiology, 1980