Evidence that the Gya, Hy and JoaAntigens Belong to the Dombrock Blood Group System

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Abstract
Ten red cell samples lacking the high incidence Gyaantigen were found to have the previously undescribed Do(a‐b‐) phenotype. Fifteen Hy‐ red cell samples were Do(a‐b+) with weak expression of Doband 6 Jo(a‐) red cell samples were Do(a+) with weak expression of Doa. Five of the 6 Jo(a‐) samples had extremely weak expression of Dob. The sixth Jo(a‐) was Do(b‐). Immune precipitates were prepared from radio‐iodinated antigen‐positive red cells with anti‐Gya, ‐Hy, ‐Doaand Dob. Immunoblotting of these immune precipitates with affinity‐purified anti‐Gyaand anti‐Dobunder non‐reducing conditions revealed similar broadly migrating bands of Mr 48,700–59,750, suggesting that the Doaand Dobantigens are carried on the same glycoprotein as Gyaand Hy. The phenotypically associated high incidence Joaantigen has previously been shown to reside on the Gya/ Hy glycoprotein. Enzyme‐treated and chemically modified red cells tested with anti‐Doa, ‐Dob, ‐Gya, ‐Hy and ‐Joagave the same pattern of reactivity. We propose that Gya, Hy and Joabecome part of the Dombrock blood group system and that, henceforth, the Gya/Hy‐active glycoprotein be renamed the Dombrock‐active glycoprotein. The Gy(a‐) Hy‐ Jo(a‐) phenotype constitutes the ‘null’ phenotype within the Dombrock system.
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