In-vitro concentration of azithromycin in human phagocytic cells

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Abstract
The In-vitro intraphagocytic uptake and retention of azithromydn in both human polymorphonuclear leucocytes (PMN) and alveolar macrophages was measured by an improved high-performance liquid chromatography (HPLC method that was approximately three-fold more sensitive than previous methods. Azithromycin was accumulated in PMN and alveolar macrophages (about 300-fold), with maximum uptake being obtained after incubation for 60 min. Azithromydn was eliminated only partially from the cells during the washing process, and was released slowly during re-incubation of phagocytic cells in antibiotic-free medium. This intracellular retention distinguishes azithromydn from most of the macrolides and quinolones which, in spite of high I/E ratios, are released rapidly from cells.

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