Isozyme characterization of cattle (Bos taurus) and American buffalo (Bison bison) cell cultures
- 1 August 1978
- journal article
- research article
- Published by Wiley in Animal Blood Groups and Biochemical Genetics
- Vol. 9 (3) , 175-179
- https://doi.org/10.1111/j.1365-2052.1978.tb01431.x
Abstract
Towards identifying cell contamination in cultures 4 Bovidae cell lines [BEK-1 (bovine embryonic kidney), MDBK (Madin-Darby bovine kidney), Bu (buffalo lung fibroblast) and EBTr (bovine trachea cell)] were characterized enzymatic biochemical markers. Of 15 enzymatic systems, 3 (adenosine deaminase (Ada), phosphoglucomutase (Pgm) and nucleoside phosphorylase (Np)) were polymorphic and suitable for biochemical identification of each cell line. The Bu cell line has a Np phenotypic pattern which could be distinctive of the B. bison species.Keywords
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