Is the adrenal angiotensin receptor angiotensin II — or angiotensin III like?

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Abstract
To determine whether the adrenal receptor is primarily directed at angiotensin [A] II or AIII the following in vitro experiments were performed examining aldosterone responsiveness in isolated rat glomerulosa cells. Cells exposed to increasing doses (2.4 .times. 10-10 M-2.4 .times. 10-6 M) of AII or AIII were significantly more responsive to AII (AII ED50 was 6.3 .times. 10-10 M vs. AIII ED50 4.6 .times. 10-9 M P < 0.001). Octapeptide analogs (Sar1, Ala8 and Asn1, Ala8), while demonstrating different inhibitory potencies relative to each other, were equally effective in blocking AII vs. AIII stimulation. The heptapeptide analogs (des1 Ala8 and des1 Ile8) however, inhibited AIII stimulation preferentially (P < 0.01). The 8 alanine octapeptide analogs were better inhibitors of both AII and AIII stimulation than the 8 alanine heptapeptide analog. High performance liquid chromatography analysis indicated that AII and AIII were being degraded at the same rate during the incubation procedure. The angiotensin adrenal receptor evidently is an AII receptor.