Studies on Mold Dextranases

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Abstract
Penicillium luteum ATCC9644 was found to produce large amounts of extracellular dextranase [EC 3.2.1.11] when grown aerobically in a medium containing 1% dextran, 0.2% each of NANO3, K2HPO4 and yeast extracts together with trace amounts of inorganic salts. An electrophoretically homogeneous prepartion of the dextranase was obtained from the culture broth by acetone precipitation, sequential chromatographics on DEAE- and CM-cellulose columns and by gel-filtration on Bio Gel P-150. The enzyme was most active at pH values between 4 and 6 in 10 min reaction and stable over the range of pH 3.5 to 7.0 at 30°C for 24 hr. Substrate specificity experiments indicated that the dextranase hydrolyzed a series of isomaltodextrins, dextran and its derivatives, but was completely inert against amylopectin, pullulan, panose and isomaltosylmaltose, all of which contained α-1, 6-glucosidic linkage at the branching point from main glucose chain of α-1, 4-glucosidic linkages.

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