Sulfide dehydrogenase is identical with the SoxB protein of the thiosulfate‐oxidizing enzyme system of Paracoccus denitrificans GB17
- 15 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (1) , 61-65
- https://doi.org/10.1016/0014-5793(94)00732-2
Abstract
Thiosulfate induced cells of Paracoccus denitrificans GB17 oxidize thiosulfate and sulfide to sulfate. A mutant carrying a Tn5-mob insertion in the soxB gene is unable to oxidize thiosulfate or sulfide suggesting a linkage of both activities. To test this assumption we have separated the components of the thiosulfate-oxidizing enzyme system of the wild-type by ion exchange chromatography. The SoxB protein coeluted with a highly active sulfide dehydrogenase. Analysis by polyacrylamide gel electrophoresis revealed one major protein of M r 32k. Thus, the SoxB protein appeared to be identical with sulfide dehydrogenase.Keywords
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