Lymphocyte calmodulin and its participation in the stimulation of T lymphocytes by mitogenic lectins
- 1 January 1992
- journal article
- Published by Wiley in Biology of the Cell
- Vol. 75 (1) , 55-59
- https://doi.org/10.1016/0248-4900(92)90124-j
Abstract
Summary— Calmodulin was purified from human tonsillar lymphocytes utilizing calcium‐dependent binding of calmodulin to fluphenazine‐Sepharose. The molecular weight and phosphodiesterase activation of the lymphocyte calmodulin were very similar to those of purified bovine brain calmodulin. Trifluoperazine (TFP), a calmodulin inhibitor, suppressed lymphocyte stimulation as assessed by 3H‐thymidine incorporation into DNA of lectin‐stimulated lymphocytes. TFP had no effect on the early 45Ca2+ uptake induced by mitogenic lectins, although this latter was inhibited by verapamil which also suppressed the 3H‐thymidine incorporation. The results are in keeping with the interpretation that the inhibition of T cell stimulation by TFP was not due to suppression of Ca2+ uptake, but due to inactivation of Ca2+‐calmodulin complex which might be formed subsequent to Ca2+ entry into the cell.Keywords
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