Rapid Destruction of the PFS Form of Phytochrome by a Substance in Extracts of Pisum Tissue

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Abstract
Crude aqueous extracts of etiolated Pisum tissue contain a substance that almost instantaneously destroys phytochrome in the Pfr [far-red-absorbing] but not in the Pr [red-absorbing] form. This Pfr-killer is separable from phytochrome by gel-filtration, is soluble in n-butanol but not in petroleum ether, and is probably inactivated by reaction with Pfr Avena extracts yield no Pfr-klller. The physiological significance of these results is doubtful, but they illuminated several technical points concerning in vitro phytochrome assays. They cast doubt on studies of comparative phytochrome content by extraction unless the Pr state is maintained from homogenization through purification and assay.

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