ENZYMATIC REDUCTION OF SELENITE

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Abstract
Nickerson, Walter J. (Rutgers, The State University, New Brunswick, N.J.) and Giuseppe Falcone . Enzymatic reduction of selenite. J. Bacteriol. 85: 763–771.—1963. Extraction of boiled yeast extract with n -hexane removed its ability to restore selenite-reducing capacity in dialyzed enzyme preparations obtained from baker's yeast or from Candida albicans . Menadione or thiodione substituted for the quinone(s) extracted by n -hexane. Active components of the water-soluble, dialyzable fraction of yeast extract included: glucose-6-phosphate, triphosphopyridine nucleotide, and glutathione. Selenite appears to be bound to protein through vicinal thiol groups, and to be released therefrom as metallic selenium after accepting four electrons.