Association of iron-protoporphyrin-IX (hemin) with myosins
- 18 June 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 266 (1-2) , 9-12
- https://doi.org/10.1016/0014-5793(90)81493-8
Abstract
Addition of myosins isolated from guinea pig heart and rabbit skeletal muscle to hemin solutions resulted in the appearance of new absorption spectra indicating association of hemin and the myosins. Binding stoichiometry based on absorption changes was found to be two hemin sites per myosin molecule. The binding constants calculated from quenching of the intrinsic fluorescence of the myosins by hemin are Ka = 7 (+/- 2) 10(6) M-1 for skeletal muscle myosin, and Ka = 3 (+/- 1) x 10(7) M-1 for heart muscle myosin. Based on these findings, myosins are suggested as potential transporters of free hemin between cell organelles.Keywords
This publication has 10 references indexed in Scilit:
- Can glutathione‐S‐transferases function as intracellular heme carriers?Journal of Cellular Biochemistry, 1989
- Association of hemin with protein 4.1 as compared to spectrin and actinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- [47] HemopexinPublished by Elsevier ,1988
- The interaction of hemin with skeletal muscle actinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- The role of glutamine in regulation of ammonium transport in Azotobacter vinelandiiArchives of Biochemistry and Biophysics, 1984
- Transitions in human atrial and ventricular myosin light-chain isoenzymes in response to cardiac-pressure-overload-induced hypertrophyBiochemical Journal, 1982
- The influence of soluble binding proteins on lipophile transport and metabolism in hepatocytesBiochemical Journal, 1981
- Electrophoretic analysis of multiple forms of rat cardiac myosin: Effects of hypophysectomy and thyroxine replacementJournal of Molecular and Cellular Cardiology, 1978
- Transfer of heme from heme-albumin to hemopexinBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Transfer of Heme from Mitochondria in Rat Liver CellsCanadian Journal of Biochemistry, 1972