New insights into sequence recognition process of esperamicin A1 and calicheamicin .gamma.1I: Origin of their selectivities and "induced fit" mechanism
- 1 May 1993
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 32 (17) , 4622-4627
- https://doi.org/10.1021/bi00068a020
Abstract
This study addresses the DNA sequence recognition event of the enediyne antibiotics esperamicin A1 and calicheamicin gamma 1I by the use of synthetic DNA oligomers, salt effects, and circular dichroism studies. The results reported here provide several important insights: (1) esperamicin A1 requires a purine/pyrimidine trimer in host DNA for favorable interaction, (2) the sequence selectivity of esperamicin C is an origin of esperamicin A1 and calicheamicin gamma 1I selectivities, (3) in the target recognition by esperamicin C, its total structure and hydrophobicity are important, and (4) the binding of hydrophobic esperamicin to DNA duplex induces dehydration and conformational change of the host DNA. The specific sequence recognition process of esperamicin/calicheamicin has been discussed.Keywords
This publication has 47 references indexed in Scilit:
- Product analyses in DNA strand scission by antitumor antibiotic elsamicin ABiochemical and Biophysical Research Communications, 1992
- Selective DNA cleavage by elsamicin A and switch function of its amino sugar groupBiochemistry, 1991
- Structure of the B-DNA decamer C-C-A-A-C-G-T-T-G-G and comparison with isomorphous decamers C-C-A-A-G-A-T-T-G-G and C-C-A-G-G-C-C-T-G-GJournal of Molecular Biology, 1991
- DNA CONFORMATION AND PROTEIN BINDINGAnnual Review of Biochemistry, 1989
- Calicheamicin γ 1 I and DNA: Molecular Recognition Process Responsible for Site-SpecificityScience, 1989
- Helix Geometry, Hydration, and G ⋅ A Mismatch in a B-DNA DecamerScience, 1987
- Calichemicins, a novel family of antitumor antibiotics. 1. Chemistry and partial structure of calichemicin .gamma.1IJournal of the American Chemical Society, 1987
- The unusual conformation adopted by the adenine tracts in kinetoplast DNACell, 1987
- Structure of a B-DNA dodecamerJournal of Molecular Biology, 1981
- Conformational changes of deoxyribonucleic acid and polydeoxynucleotides in water and ethylene glycolBiochemistry, 1971