Rapid Freezing and Thawing of Whole Blood
- 1 December 1955
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 90 (3) , 587-589
- https://doi.org/10.3181/00379727-90-22106
Abstract
The necessary extreme rates of heat transfer were obtained with whole blood by spraying it through a plastic capillary onto the surface of liquid N. Reconstitution is achieved by sprinkling the frozen pellets into warm saline or plasma. Heparinized whole blood so treated suffers 15-20% hemolysis. Addition of dextrose, designed to reduce crystallization velocities and thus reduce crystal size, enables a recovery of 98% of the erythrocytes. Preliminary human transfusions of Cr51 tagged red cells show that erythrocytes can survive normally in the circulation after rapid freezing and thawing. Storage at -60[degree]C causes a slow increase in hemolysis on thawing. Storage in liquid N is. expected to be virtually indefinite.Keywords
This publication has 1 reference indexed in Scilit:
- Het mechanism of the protective action of glycerol against haemolysis by freezing and thawingPublished by Elsevier ,2003