Comparison of Washing and Swabbing Procedures for Collecting Genital Fluids To Assess Cervicovaginal Shedding of Herpes Simplex Virus Type 2 DNA
Open Access
- 1 June 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (6) , 2662-2664
- https://doi.org/10.1128/jcm.41.6.2662-2664.2003
Abstract
Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.Keywords
This publication has 12 references indexed in Scilit:
- Polymerase chain reaction for Y chromosome to detect semen in cervicovaginal fluid: a prerequisite to assess HIV-specific vaginal immunity and HIV genital sheddingAIDS, 2001
- Comparison of Techniques for HIV-1 RNA Detection and Quantitation in Cervicovaginal SecretionsJAIDS Journal of Acquired Immune Deficiency Syndromes, 2001
- Comparison of Techniques for HIV-1 RNA Detection and Quantitation in Cervicovaginal SecretionsJAIDS Journal of Acquired Immune Deficiency Syndromes, 2001
- Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group.2000
- Interactions between Herpes Simplex Virus Type 2 and Human Immunodeficiency Virus Type 1 Infection in African Women: Opportunities for InterventionThe Journal of Infectious Diseases, 2000
- Selection of Drug‐Resistant Variants in the Female Genital Tract of Human Immunodeficiency Virus Type 1–Infected Women Receiving Antiretroviral TherapyThe Journal of Infectious Diseases, 2000
- A monoclonal blocking EIA for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in AfricaJournal of Virological Methods, 2000
- Genital Herpes and Public HealthJAMA, 2000
- Typing and strain differentiation of clinical Herpes simplex virus type 1 and 2 isolates by Polymerase Chain reaction and subsequent restriction fragment length polymorphism analysisZentralblatt für Bakteriologie, 1994
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985