Determination of free myo-inositol in milk and infant formula by high-performance liquid chromatography
- 1 January 1994
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 119 (3) , 397-402
- https://doi.org/10.1039/an9941900397
Abstract
A high-performance liquid chromatographic method is described for the determination of supplemental myo-inositol in infant formulas based on cows' milk. The technique incorporates pre-column derivatization with phenylisocyanate followed by reversed-phase gradient chromatography and ultraviolet detection. The protocol was also applied to a survey of free inositol at endogenous levels in the milk of cows, goats and humans. Nutritional preparations containing inositol at pharmacological levels are also amenable to a simplified isocratic analysis.Keywords
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