Transpositional behavior of the maize En/Spm element in transgenic tobacco
Open Access
- 1 May 1989
- journal article
- research article
- Published by Wiley in The EMBO Journal
- Vol. 8 (5) , 1315-1321
- https://doi.org/10.1002/j.1460-2075.1989.tb03511.x
Abstract
The transposable element Enhancer (En/Spm) of Zea mays encodes the functions necessary for transposition of members of the En‐I family of elements. En constructs were introduced into tobacco and transposition was demonstrated by cloning and DNA sequence analysis. Transcriptional analysis of En transgenic plants revealed 2.5 and 6 kb En homologous transcripts similar in size to those found in maize, in addition to aberrant splice products. Progeny analysis of a primary transformant indicates En transpositional activity after meiosis. In these progeny somatic variegation, at the NPT II gene, was demonstrated to be caused by En excision. Two re‐insertions of En were analyzed and found to reside in unique copy DNA while another was found in middle repetitive DNA.Keywords
This publication has 27 references indexed in Scilit:
- Organization of the Tgm family of transposable elements in soybean.Genetics, 1988
- Genetic and Molecular Analysis of the Spm-dependent a-m2 Alleles of the Maize a LocusGenetics, 1987
- Transposition of the maize controlling element “Activator” in tobaccoProceedings of the National Academy of Sciences, 1986
- Transposition in plants: a molecular modelThe EMBO Journal, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Isolation of the transposable maize controlling elements Ac and DsCell, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens.The EMBO Journal, 1983
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Intranuclear systems controlling gene action and mutation.1956