Chloroplast Isolation in Nonaqueous Media.

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Abstract
A technique is described whereby chloroplasts can be isolated from lyophilized leaves without the use of an aqueous medium. Such chloroplasts do not contact liquid water at any time during their isolation. Consequently water soluble enzymes are not leached from them. Chloroplasts so isolated have a high aldolase activity and are associated with at least 54% of the cell phosphorylase. The nonaqueous method of preparing chloroplasts, while suffering from difficulties associated with the use of lipid solvents, promises to be useful as a supplement to the aqueous methods in the study of the intracellular distribution of enzymes in leaves.