o-Phenylenediamine-Modified Carbon Fiber Electrodes for the Detection of Nitric Oxide
- 1 August 1996
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 68 (15) , 2621-2628
- https://doi.org/10.1021/ac960093w
Abstract
Nitric oxide (NO•) sensors were prepared using o-phenylenediamine (o-PD) and Nafion to modify the surface of 30 μm diameter carbon fiber electrodes. These electrodes were compared with nickel porphyrin-type NO• sensors that have already been described. High-speed chronoamperometry, amperometry, and differential pulse voltammetry were used to compare the performance of sensors modified with various combinations of Nafion, o-PD, or nickel(II) meso-tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin (Ni-TMPP), in order to determine which electrodes had the most sensitivity and selectivity for NO•. Our findings showed that electrodes treated with Nafion first, followed by o-PD, were very sensitive to NO•, with a detection limit of 35 ± 7 nM. In addition, o-PD electrodes were also very selective against ascorbate (>600:1), dopamine (>300:1), and nitrite (>900:1). Moreover, in the range of 0−6 μM NO•, o-PD electrodes displayed excellent linearity (R2 ≥ 0.997). In contrast, Ni-TMPP electrodes (with Nafion) had significantly poorer detection limits (76 ± 12 nM) and were less selective against dopamine (o-PD electrodes (R2 ≥ 0.911). Finally, we tested the in vitro and in vivo performance of the o-PD electrode in terms of its ability to detect NO• release from isolated rat renal arterioles and to measure NO• diffusion in the extracellular space of the rat brain.Keywords
This publication has 28 references indexed in Scilit:
- Measurement in vitro of Human Plasma Glycerol with a Hydrogen Peroxide Detecting Microdialysis Enzyme ElectrodeAnalytical Chemistry, 1994
- Models of the diffusional spread of nitric oxide: Implications for neural nitric oxide signalling and its pharmacological propertiesNeuropharmacology, 1994
- PRODUCTION OF NITRIC OXIDE IN ANAPHYLAXIS IN RABBITSShock, 1994
- A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis techniqueNeuroscience Letters, 1994
- Diffusion of Nitric Oxide in the Aorta Wall Monitored in Situ by Porphyrinic MicrosensorsBiochemical and Biophysical Research Communications, 1993
- Nitric Oxide Measured by a Porphyrinic Micro Sensor in Rat Brain after Transient Middle Cerebral Artery OcclusionJournal of Cerebral Blood Flow & Metabolism, 1993
- Nitric oxide as a signal in blood vesselsTrends in Biochemical Sciences, 1992
- A novel neuronal messenger molecule in brain: The free radical, nitric oxideAnnals of Neurology, 1992
- Regional effects of aging on dopaminergic function in the Fischer-344 ratNeurobiology of Aging, 1992
- Transient infrared emission spectroscopy by pulsed laser excitationAnalytical Chemistry, 1989