Ploidy in bone marrow cells from healthy donors: a MAC (morphology antibody chromosomes) study
- 1 January 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 86 (1) , 203-206
- https://doi.org/10.1111/j.1365-2141.1994.tb03279.x
Abstract
The ploidy of human bone marrow cells belonging to the megakaryocytic, granulocytic‐monocytic and erythrocytic lineages was studied by in situ hybridization using the biotin‐labelled Y chromosome‐specific DNA probe pY431 and the chromosome 1‐specific probe pUC1.77 on cells identified morphologically and immunologically by the MAC procedure. Cells of the granulocytic‐monocytic and erythrocytic lineages were seen to be 2N in ploidy, whereas the ploidy of the megakaryocytic lineage ranged from 2N to 32N, with the ploidy classes 4N and 8N being predominant. The frequency of megakaryocytes with 2N chromosomes was also high.Keywords
This publication has 8 references indexed in Scilit:
- Analysis of phenotype and genotype of individual cells in neoplasmsCancer Genetics and Cytogenetics, 1993
- Immunophenotyping of aneuploid cellsCancer Genetics and Cytogenetics, 1989
- Flow cytometric analysis of normal human megakaryocytesBlood, 1988
- Separation of human megakaryocytes by state of differentiation on continuous gradients of Percoll: size and ploidy analysis of cells identified by monoclonal antibody to glycoprotein IIb/IIIaBlood, 1986
- The relation of megakaryocyte ploidy to platelet volumeAmerican Journal of Hematology, 1984
- Isolation and Characterization of Normal Human MegakaryocytesBritish Journal of Haematology, 1980
- Cloning of human satellite III DNA: different components are on different chromosomesNucleic Acids Research, 1979
- FLUOROMETRIC MEASUREMENT OF DEOXYRIBONUCLEIC ACID IN BONE MARROW CELLS THE MEASUREMENT OF MEGAKARYOCYTE DEOXYRIBONUCLEIC ACIDJournal of Histochemistry & Cytochemistry, 1972