Human Platelet-Derived Growth Factor Preparations Contain a Separate Activity which Potentiates Follicle- Stimulating Hormone-Mediated Induction of Luteinizing Hormone Receptor in Cultured Rat Granulosa Cells: Evidence for Transforming Growth Factor-β*

Publisher Website
Google Scholar
Abstract
The ability of platelet-derived growth factor (PDGF) preparations to potentiate FSH-mediated LH receptor induction in rat granulosa cell cultures was shown to be due to a component distinct from PDGF. Purification of heat-treated platelet lysate by carboxymethyl-Sephadex C-50 and Cibacron blue-Sepharose chromatography, followed by Bio-Gel P-60 chromatography, resulted in the separation of two activities: 1) a growth-promoting activity, P60-PDGF, defined on the basis of increased DNA synthesis in BALB/c-3T3 cells, and 2) a differentiation- promoting activity which enhanced FSH-dependent LH receptor induction in granulosa cells. On the basis of electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gels, inhibition of tritiated thymidine uptake by epithelial cells, and attenuation of LH/hCG receptor expression in the presence of antitransforming growth factor-β (anti-TGFβ) immunoglobulin G, the differentiation-promoting component of the preparations appears to be TGFβ. The Bio-Gel fractions that contained TGFβ did not stimulate LH receptor induction or cAMP production in the absence of FSH. PDGF prepared free of TGFβ did not potentiate receptor induction. We conclude, therefore, that the differentiative effects of PDGF previously described in this system are due to TGFβ. (Endocrinology123: 2003–2008, 1988)

This publication has 23 references indexed in Scilit: