Hepatic iodothyronine 5′-deiodinase. The role of selenium
- 30 November 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 272 (2) , 537-540
- https://doi.org/10.1042/bj2720537
Abstract
Selenium (Se) deficiency decreased by 8-fold the activity of type I iodothyronine 5''-deiodinase (ID-I) in hepatic microsomal fractions from rats. Solubilized hepatic microsomes from rats injected with 75Se-labelled Na2SeO3 4 days before killing were found by chromatography on agarose gels to contain a 75Se-containing fraction with ID-I activity. PAGE of this fraction under reducing conditions, followed by autoradiography, revealed a single 75Se-containing protein (Mr 27,400 .+-. 300). This protein could also be labelled with 125I-bromoacetyl reverse tri-iodothyronine, an affinity label for ID-I. The results suggest that hepatic ID-I is a selenoprotein or has an Se-containing subunit essential for activity.This publication has 14 references indexed in Scilit:
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