Topographical studies on intestinal microvillous leucine β-naphthylamidase on the outer membrane surface
- 1 December 1978
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 39 (4) , 285-296
- https://doi.org/10.1007/bf01869895
Abstract
The location of leucine β-naphthylamidase on the outer surface of the microvillous membrane of rabbit small intestine was examined by analyzing the interaction of antibodies against leucine β-naphthylamidase or another microvillous enzyme, sucrase-isomaltase complex, with microvillous vesicles having different relative amounts of these enzymes, in respect to vesicle agglutination, inhibition of enzyme activity, and electron-microscopic morphology. The results obtained indicate that leucine β-naphthylamidase, or at least its antigenic sites, protrude about 10 nm from the outer surface of the microvillous membrane.Keywords
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