Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein
Open Access
- 1 July 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (13) , 2620-2626
- https://doi.org/10.1093/nar/25.13.2620
Abstract
Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies.Keywords
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