Effects of unsaturated fatty acid deprivation on neutral lipid synthesis in Saccharomyces cerevisiae

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Abstract
The effects of unsaturated fatty acid deprivation on lipid synthesis in S. cerevisiae strain GL7 were determined by following the incorporation of [14C]acetate. Compared to yeast cells grown with oleic acid, unsaturated fatty acid-deprived cells contained 200 times as much 14C label in squalene, with correspondingly less label in 2,3-oxidosqualene and 2,3;22,23-dioxidosqualene. Cells deprived of either methionine or cholesterol did not accumulate squalene, demonstrating that the effect of unsaturated fatty acid starvation on squalene oxidation was not due to an inhibition of cell growth. Cell deprived of olefinic supplements displayed additional changes in lipid metabolism: an increase in 14C-labeled diacylglycerides, a decrease in 14C-labeled triacylglycerides, and increased levels of 14C-labeled decanoic and dodecanoic fatty acids. The changes in squalene oxidation and acylglyceride metabolism in unsaturated fatty acid-deprived cells were readily reversed by adding oleic acid. Pulse-chase studies demonstrated that the [14C]squalene and 14C-labeled diacylglycerides which accumulated during starvation were further metabolized when cells were resupplemented with oleic acid. Unsaturated fatty acids are essential for normal lipid metabolism in yeasts.

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