TRANSFER OF MOUSE IgG2 PRODUCTION BY IgM‐BEARING SPLEEN CELLS SEPARATED BY A FLUORESCENCE‐ACTIVATED CELL SORTER

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Abstract
The purpose of the present study was to determine whether at least some splenic B lymphocytes can switch from the synthesis of one isotype of immunoglobulin to another during B cell differentiation. The experimental system involved the transfer of characterized cell suspensions between allotype congenie strains of mice followed by analysis in the recipient for donor type immunoglobulin production. Donor splenic lymphocytes were incubated with specific fluorescent labelled anti- antiserum and passed through the Los Alamos fluorescence-activated cell sorter; -depleted cell suspensions were transferred into sublethally irradiated congenic recipients and the amount of donor type immunoglobulin of IgG2 type was measured at weekly intervals. The results demonstrated that at least some cells hearing membrane bound IgM can differentiate in vivo into IgG2-secreting cells, although not all IgG2-secreting cells have been recently derived from IgM positive precursors.

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