Occurrence of Coenzyme Q12and Coenzyme Q13in Facultative Methanol-oxidizing Bacteria

Abstract
Pseudomonas C45 is the mutant of facultative methanol-oxidizing bacteria, Pseudomonas N842, and this mutant accumulated CoQ10 and CoQ11, Minor components of CoQ faomologs were studied and four homologs were isolated. Two of them with higher Rf values than that of CoQ10 on the reversed-phase chromatogram were identified as CoQ8 and CoQ9. One CoQ homolog with lower Rf value than that of CoQ11 was purified to crystaline state and identified as CoQ12 by chemical properties and NMR, mass and IR spectrometries. The fourth CoQ homolog with the lowest Rf value was identified as CoQ13, which was novel in nature. CoQ8 through CoQ13 were also detected in similar methanol-oxidizing bacteria by high performance liquid chromatography.

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