Two Migration Inhibitory Factors with Different Chromatographic Behavior and Isoelectric Points
Open Access
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 118 (6) , 2015-2019
- https://doi.org/10.4049/jimmunol.118.6.2015
Abstract
Migration inhibitory factor (MIF), produced by stimulation of guinea pig lymph node cells with concanavalin A, was fractionated by Sephadex G-100 gel filtration, sucrose density gradient electrophoresis, and isoelectrofocussing. Two distinct species were identified and separated. One, pH 3-MIF, has an isoelectric point of 3.0 to 4.5 and elutes from Sephadex G-75 columns with molecules having an apparent m.w. of 65,000 (Kd of 0.05 to 0.12). The other, pH 5-MIF, has an isoelectric point of 5.0 to 5.5 and elutes with molecules having an apparent m.w. between 25,000 and 43,000 (Kd of 0.15 to 0.23).This publication has 2 references indexed in Scilit:
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