Preparation of theProteus MirabilisBacterial Electrode For the Determination of Urea and It's Clinical Applications
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 22 (1) , 1-13
- https://doi.org/10.1080/00032718908051181
Abstract
A bacterial electrode for the determination of urea has been constructed by immobilizing the Proteus mirabilis on a carbon dioxide gas-sensor. the electrode gave a Nernstian behaviour between 7.0 × 10−4 and 3.0 × 10−2 M urea with a slope of 46 mV/decade in pH 6.80, 0.1M phosphate buffer at 30°C. the important interferences were L-asparagine, cytosine, inositol and phenol, and most inorganic salts reacted as the inhibitor. This electrode showed little change in the response and linear rane for 7 days, and could also be used in the linear range because the electrode had good reproducibility even after this. This device could be used as easily and exactly as a spectrophotometric method in clinical applications.Keywords
This publication has 17 references indexed in Scilit:
- An activated enzyme electrode for creatinineAnalytica Chimica Acta, 1976
- Highly selective enzyme electrode for 5'-adenosine monophosphateAnalytical Chemistry, 1976
- A urea-sensing membrane electrode for whole blood measurementsAnalytica Chimica Acta, 1975
- Membrane Electrode Probes for Biological SystemsScience, 1975
- A Urea Electrode Based on the Ammonia ProbeAnalytical Letters, 1973
- Determination of glucose, urea and penicillin using enzyme-pH-electrodesBiochimica et Biophysica Acta (BBA) - General Subjects, 1973
- An electrode for the determination of glutamineAnalytica Chimica Acta, 1971
- New enzyme electrode probes for d-amino acids and asparagineAnalytica Chimica Acta, 1971
- Enzyme electrode for the substrate ureaJournal of the American Chemical Society, 1970
- Urea-specific enzyme electrodeJournal of the American Chemical Society, 1969