Spectrophotofluorometric and gas-liquid chromatographic methods for the estimation of mexiletine (Kö 1173) in plasma and urine
- 1 July 1973
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 25 (7) , 550-553
- https://doi.org/10.1111/j.2042-7158.1973.tb09155.x
Abstract
Methods are presented for the spectrophotofluorometric and gas-liquid chromatographic determination in plasma and urine of the anti-arrhythmic compound, mexiletine (Kö 1173). Both methods involve extraction of the drug from alkaline plasma with ether. In the spectrophotofluorometric method the compound is re-extracted into 0ṁ05n HCl and emission intensity determined at 300 nm with activation at 228 nm. In the chromatographic method the drug is acylated during evaporation of the ether. Both butyryl and acetyl derivatives could be used. Use of a nitrogen-sensitive detector increased the sensitivity and selectivity of the method and allowed more rapid analyses. There was good agreement between results obtained by spectrophotofluorometric and chromatographic methods.Keywords
This publication has 1 reference indexed in Scilit:
- The effect of Kö 1173, a new anticonvulsant agent on experimental cardiac arrhythmiasBritish Journal of Pharmacology, 1972