Determination of the Solution Conformation of Adenosine 2′:3′‐Monophosphate by Nuclear Magnetic Resonance with Lanthanide Probes

Abstract
The aqueous solution conformation of adenosine 2′:3′‐monophosphate at pH 2.5 has been determined by a nuclear magnetic resonance method utilizing lanthanide ions as shift and relaxation probes. The ribose conformation is best described as a rapid equilibrium of 2′‐endo(3′‐exo) and 3′‐endo(2′‐exo) conformations in a ratio of approximately 2 to 1. The orientation of the base relative to to ribose is restricted to a narrow range about χcn=−70°.

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