Biotinylation of transducin and G0 from bovine brain
- 1 June 1991
- journal article
- Published by Springer Nature in Protein Journal
- Vol. 10 (3) , 325-332
- https://doi.org/10.1007/bf01025631
Abstract
A method is described for modifying G proteins with biotin. With transducin, better results were obtained with the amino group-specific derivative BXNHS (biotinyl-ε-aminocaproic acid N-hydroxysuccinimide ester) as compared to the -SH-group specific reagent MBB (maleimidobutyrylbiocytin). Modification occurred under conditions preserving functional activity: Interaction of the biotinylated transducin with rod outer segment membranes was shown by its light-dependent association and by a GTPγS-binding assay. G0 from bovine brain was also biotinylated under conditions preserving its activity. Biotinyl-α0 was shown to bind to a streptavidin Sepharose matrix. Biotinyl-G proteins, therefore, are proposed as tools for extracting proteins (receptors and effector systems), which interact under specific conditions with G proteins.Keywords
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