Biosynthesis of Cytokinins in Cytokinin-Autotrophic Tobacco Callus
- 1 March 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (3) , 658-660
- https://doi.org/10.1073/pnas.70.3.658
Abstract
A cytokinin-autotrophic strain of tobacco callus contained cytokinin-active compounds with chromatographic mobilities on Sephadex LH-20 corresponding to ribosylzeatin, zeatin, and 6-Δ 2 -isopentenylaminopurine. Zeatin, the apparent major cytokinin, was estimated to be present at a concentration of 10 -4 μmol/kg of tissue. Cytokinin-autotrophic callus supplied with [ 14 C]adenine produced radioactive components with the same chromatographic properties as zeatin and 6-Δ 2 -isopentenylaminopurine. These components were not obtained from cytokinin-dependent tissue supplied with [ 14 C]adenine in the same manner.Keywords
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