Detection of Leishmania within Sand Flies by Kinetoplast DNA Hybridization
- 1 November 1988
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 39 (5) , 434-439
- https://doi.org/10.4269/ajtmh.1988.39.434
Abstract
A kinetoplast DNA hybridization probe method was used to detect Leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, Endotrypanum. Eighty-one sand flies (74 Lutzomyia umbratilis, 1 Lu. anduzei, and 6 Lu. shannoni) collected outside Manaus, Brazil were dissected. Forty-four of these were found to be infected with flagellates, and 2 hybridized with a Leishmania braziliensis probe. Thirty-three of sixty-one flies reprobed with an Endotrypanum probe were positive.This publication has 4 references indexed in Scilit:
- Kinetoplast DNA minicircles: regions of extensive sequence divergence.Proceedings of the National Academy of Sciences, 1987
- Flagellate Infections of Brazilian Sand Flies (Diptera: Psychodidae): Isolation In Vitro and Biochemical Identification of Endotrypanum and LeishmaniaThe American Journal of Tropical Medicine and Hygiene, 1985
- The Development of a Solid Phase Radioimmunoassay for the Detection of Leishmanial Parasites in the Sand FlyThe American Journal of Tropical Medicine and Hygiene, 1985
- Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.Proceedings of the National Academy of Sciences, 1982