Studies on the Site of Synthesis of Transcobalamin-II

Abstract
The liver, kidneys, and stomach were studied to determine the site of transcobalamin-n synthesis. The kidney is not the site since total nephrectomy had no effect on the reappearance of TC-n after saturation of TC-II with B12. Total gastrectomy also had little effect on TC-II levels or rate of synthesis. The liver proved to be the site of TC-n synthesis because destruction of liver with CC1 resulted in a decrease of serum TC-n and unsaturated TC-n in the blood after saturation with cyanocobalamin was markedly low in the CC1 treated animals. TC-n was also synthesized by the perfused rat liver.

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