Preliminary investigation of the characteristics of fish lymphocytes separated on a Percoll discontinuous gradient

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Abstract
This investigation was carried out to determine if lymphocyte functional heterogeneity could be demonstrated in teleosts on the basis of density difference. The lymphocytes separated into two fractions on a Percoll discontinuous gradient. The less dense lymphocytes separated at 1.056gml−1 and contained both moderately villous and smooth lymphocytes, whereas the more dense lymphocytes separated at 1.07 g ml−1 and were predominantly moderately villous lymphocytes. The moderately villous lymphocytes had a higher mitochondria content than the smooth lymphocytes. Lymphocytes separating in the 1.056g ml−1 layer were more responsive to stimulation with polyclonal mitogen (PHA) than the lymphocytes separating in the 1.07 g ml−1 layer. This indicates that the lymphocytes separated according to either cell lineage or maturity.