Characterization of immune responses following intramuscular DNA immunization with the MOMP gene ofChlamydia trachomatismouse pneumonitis strain
Open Access
- 1 February 1999
- journal article
- research article
- Published by Wiley in Immunology
- Vol. 96 (2) , 314-321
- https://doi.org/10.1046/j.1365-2567.1999.00682.x
Abstract
Studies were carried out to characterize the cellular and humoral immune responses evoked by intramuscular DNA vaccination with the major outer membrane protein (MOMP) gene of Chlamydia trachomatis mouse pneumonitis strain. The data demonstrate that DNA vaccinated mice develop antigen-specific delayed-type hypersensitivity, lymphocyte proliferation and interferon-γ (IFN-γ) production. Serum antibody responses (mainly immunoglobulin G2a; IgG2a) were evoked in two-thirds of the mice. We conclude that intramuscular DNA immunization with the MOMP gene evokes cellular and humoral immune responses suggestive of a T helper 1 (Th1) bias.Keywords
This publication has 32 references indexed in Scilit:
- Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene ImmunizationScience, 1996
- Immunity to Chalmydia trachomatis: lessons from a Gambian villageJournal of Medical Microbiology, 1996
- CpG motifs in bacterial DNA trigger direct B-cell activationNature, 1995
- Protective efficacy of a parenterally administered MOMP-derived synthetic oligopeptide vaccine in a murine model of Chlamydia trachomatis genital tract infection: serum neutralizing IgG antibodies do not protect against chlamydial genital tract infectionVaccine, 1995
- Partial protection against genital reinfection by immunization of guinea-pigs with isolated outer-membrane proteins of the chlamydial agent of guinea-pig inclusion conjunctivitisJournal of General Microbiology, 1993
- Heterotypic protection of mice against chlamydial salpingitis and colonization of the lower genital tract with a human serovar F isolate of Chlamydia trachomatis by prior immunization with recombinant serovar L1 major outer-membrane proteinJournal of General Microbiology, 1992
- Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogensJournal of General Microbiology, 1991
- Trachoma vaccine field trials in The GambiaEpidemiology and Infection, 1969
- Experimental inclusion conjunctivitis in man: measurements of infectivity and resistancePublished by American Medical Association (AMA) ,1965
- EXPERIMENTS WITH TRACHOMA VACCINES Experimental System Using Inclusion Blennorrh$oelig;a VirusThe Lancet, 1961