Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae
Open Access
- 30 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (3) , 472-476
- https://doi.org/10.1016/s0014-5793(99)01555-0
Abstract
The mitochondrial carrier protein for carnitine has been identified in Saccharomyces cerevisiae. It is encoded by the gene CRC1 and is a member of the family of mitochondrial transport proteins. The protein has been over‐expressed with a C‐terminal His‐tag in S. cerevisiae and isolated from mitochondria by nickel affinity chromatography. The purified protein has been reconstituted into proteoliposomes and its transport characteristics established. It transports carnitine, acetylcarnitine, propionylcarnitine and to a much lower extent medium‐ and long‐chain acylcarnitines.Keywords
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