CHICKEN FETAL INTESTINE IN TISSUE-CULTURE
- 1 January 1985
- journal article
- research article
- Vol. 44 (4) , 561-571
Abstract
A simple and reproducible method for cultivation of fetal chicken small intestine is presented. The culture was performed in a defined medium without serum. Duodena were excised from embryos at the 14th day of fetal development and cut in small segments that were maintained in culture until day 16. It could be shown that the morphology of cultured intestine resembles that of noncultured gut of corresponding age as judged by light microscopy. The increase in activity of sucrase and maltase in cultured explants is comparable with that of intestine in ovo, whereas that of alkaline phosphatase is lower than under in vivo conditions. Hormones (thyroxine, dexamethasone) influence the enzymic pattern of fetal intestine in a known manner. Therefore, the method permits maintenance of fetal intestine in tissue culture for 2 days, a period sufficient for investigation of maturation processes of intestinal mucosa.This publication has 11 references indexed in Scilit:
- Chick embryo intestine in culture: Superinduction of alkaline phosphatase and maltase activities by actinomycin DDevelopmental Biology, 1981
- Effect of sucrose refeeding on disaccharidase and aminopeptidase activities of intestinal villus and crypt cells in adult rats Evidence for a sucrose-dependent induction of sucrase in the crypt cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 1980
- Microvillous membrane vesicle accumulation in media during culture of intestine of chick embryoBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Embryonic chick intestine in organ culture: Hydrocortisone and vitamin D-mediated processesArchives of Biochemistry and Biophysics, 1979
- Morphological development of the epithelium of the embryonic chick intestine in culture: Influence of thyroxine and hydrocortisoneJournal of Anatomy, 1978
- Alkaline phosphatase and maltase activity in the embryonic chick intestine in cultureDevelopmental Biology, 1978
- Effect of various sugars on the induction of chick embryonic intestinal disaccharidases in the organ culture system.Journal of Nutritional Science and Vitaminology, 1978
- AN ALTERNATIVE MECHANISM FOR GLUTEN TOXICITY IN CŒLIAC DISEASEThe Lancet, 1976
- A Light and Electron Microscopic Study of Duodenal Epithelium of Chick Embryos Cultured in the Presence and Absence of HydrocortisoneGastroenterology, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951