The Extraction and Separation of Barley Glutelins and their Relationship to Other Endosperm Proteins

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Abstract
Procedures are described for the extraction of unmodified and alkylated barley glutelin fractions and their subsequent separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The resulting separations are considerably clearer and sharper than those previously published. One major polypeptide is present in the salt-soluble and glutelin fractions in varying proportions depending on whether or not the salt-soluble fraction is extracted in the presence of a reducing agent. There is also variation in the amount of contaminating hordein polypeptides in the glutelins and this appears to depend not only on the conditions used to extract hordein but also those used to extract the salt-soluble fraction. Finally, variation in the glutelin pattern also occurs when different denaturing agents or reducing agents are used. 1 Visiting Scientist, permanent address: U.S. Department of Agriculture, Science and Education Administration, Agricultural Research, Dept. of Agronomy Univ. of illinois, 1102 S. Goodwin Ave., Urbana, IL 61801, USA.

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