the relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. III. Expression of a single predominant isotype on primed and unprimed B cells

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Abstract
Primed and unprimed B [bone marrow-derived] cells in the spleen of (BALB/c .times. C57BL/Ka)F1 mice were tested to determine whether subpopulations that express a predominant surface Ig [immunoglobulin] isotype exist. Spleen cells were stained for surface isotypes and sorted on the fluorescence-activated cell sorter (FACS) to obtain B cells bearing predominantly IgM (.mu.p cells), IgD (.delta.p cells) or IgG (.gamma.p cells). Each population was assayed for its capacity to restore the adoptive primary and secondary anti-bovine serum albumin (BSA) antibody response in irradiated syngeneic recipients. The adoptive response restored by isotype-predominant cells was compared to that restored by isotype-positive cells (B cells bearing a given surface isotype alone or in combination with others). The .mu.p cells restore the adoptive primary and secondary IgM and IgG responses to BSA; .gamma.p cells restore only the primary and secondary IgG response. The .delta.p cells restored the adoptive secondary IgG response, but failed to restore the adoptive primary response at the cell doses tested. The .gamma.p cells but not .delta.p cells suppressed the IgM response of the .mu.+ and .delta.+ cells. The contribution of isotype-predominant cells to the adoptive primary and secondary anti-BSA response was smaller than that of B cells bearing a combination of surface isotypes. Differences in the Ig isotype pattern expressed on the surface of primed and unprimed B cells were discussed.

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