Locating and identifying posttranslational modifications by in‐source decay during MALDI‐TOF mass spectrometry
- 1 January 1999
- journal article
- Published by Wiley in Protein Science
- Vol. 8 (11) , 2487-2493
- https://doi.org/10.1110/ps.8.11.2487
Abstract
A technique is described for identifying and locating posttranslational modifications (PTMs) in peptides and proteins of known sequence by interpretation of c(n) ion signals generated by in-source decay during delayed ion extraction in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sites of phosphorylation in seven synthetic peptides were determined, as was the location of both the heme group and N,N,N-trimethyllysine in yeast cytochrome c. A semi-automated data analysis process facilitates the identification of segments of the sequence on each side of the PTM, permitting its placement at the junction of the segments and definition of the added mass. A graphical display facilitates illustration of both the location and mass of the PTM.Keywords
This publication has 16 references indexed in Scilit:
- Applications of In-Source Fragmentation of Protein Ions for Direct Sequence Analysis by Delayed Extraction MALDI-TOF Mass SpectrometryAnalytical Chemistry, 1998
- Phosphopeptide Analysis by Matrix-Assisted Laser Desorption Time-of-Flight Mass SpectrometryAnalytical Chemistry, 1996
- Selective Detection and Sequencing of Phosphopeptides at the Femtomole Level by Mass SpectrometryAnalytical Biochemistry, 1996
- Factors that influence the observed fast fragmentation of peptides in matrix-assisted laser desorptionJournal of the American Society for Mass Spectrometry, 1996
- Sequence-Specific Fragmentation of Matrix-Assisted Laser-Desorbed Protein/Peptide IonsAnalytical Chemistry, 1995
- Sequenching of peptides in a time-of-flight mass spectrometer: evaluation of postsource decay following matrix-assisted laser desorption ionisation (MALDI)International Journal of Mass Spectrometry and Ion Processes, 1994
- Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometryJournal of the American Society for Mass Spectrometry, 1993
- LC/MS and LC/MS/MS Screening for the Sites of Post-Translational Modification in ProteinsPublished by Springer Nature ,1991
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- Contributions of mass spectrometry to peptide and protein structureJournal of Mass Spectrometry, 1988