High Terminal Deoxynucleotidyl Transferase Activity in a New T-Cell Line (RPMI 8402) of Acute Lymphoblastic Leukemia Origin 2
- 1 July 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 55 (1) , 11-14
- https://doi.org/10.1093/jnci/55.1.11
Abstract
High activity of terminal deoxynucleotidyl transferase (terminal transferase) was found in a new “thymus-dependent” cell line (RPMI 8402) which is of acute lymphoblastic leukemia origin. This enzyme resembled the terminal transferase from other human cells in all its properties including Km (0.7 × 10-6 M for dGTP). The high activity of this enzyme in RPMI 8402 and fresh acute leukemia lymphoblasts, in contrast to the low activity of this enzyme reported for “thymus-independent” cells, suggested that this cell line may have originated from leukemia cells. Moreover, the high activity of terminal transferase in RPMI 8402 cells should make feasible large-scale purification of this enzyme for detailed studies.Keywords
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