Ozone Inactivation of Pseudomonas aeruginosa, Escherichia coli, Shigella sonnei and Salmonella typhimurium in Water.

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Abstract
Based on the properties of ozone as a strong germicidal agent, inactivation kinetics of Pseudomonas aeruginosa, Escherichia coli, Shigella sonnei and Salmonella typhimurium towards ozone in water were studied. The values of 90% inactivation (t90) obtained varied from 0.20 minutes (2.4 mg/L, Escherichia coli ATCC 25922) to 8.33 minutes (0.39 mg/L, Pseudomonas aeruginosa wild strain). First order inactivation kinetics with respect to both the concentrations of ozone and microorganisms were found, resulting an overall second order inactivation kinetics. The ATCC strains showed to be the most sensitive toward ozone among all. Meanwhile, the environmental isolation of Pseudomonas aeruginosa was the most resistant and Escherichia coli the most sensitive wild strain. The longest time required to achieve total inactivation was 35 minutes.