High Performance Size Exclusion Liquid Chromatography of Small Molecular Weight Peptides from Protein Hydrolysates Using Methanol as a Mobile Phase Additive

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Abstract
The separation of low molecular weight peptides according to their molecular weight has been a challenge. The earlier works using chaotropic additives in the mobile phase such as SDS or guanidium chloride failed to give a linear response for the semilogarithmic graph of molecular weight versus retention time. Here we report a mobile phase composition suited to the size exclusion separation of the peptides of molecular weight between 6000 and 250 on a TSK-SW 2000